• General Analysis→Complete Analysis for any probe-target pair including reaction ΔG^o's, ΔG^ooverall, hybridization efficiency, and formamide dissociation profile.
• ΔG^o_overall Series→ Calculate ΔG^o_overallvalues for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₁ Series→ Calculate ΔG^o₁values for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₂ Series→ Calculate ΔG^o₂values for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₃ Series→ Calculate ΔG^o₃values for a series of probes of constant length targeting a given region on the organism of interest
• Mismatch Analysis→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
• Formamide Curve Generator→Derivation of formamide dissociation profiles for multiple probe/target pairs.
• Competitor Analysis→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.

• Help→ Guidelines for mathFISH
• Links→ Links to useful websites
• Citations→List of Citations
• Contact Us→ Give us your valuable feedback

mathFISH:

A web tool for the computational evaluation of RNA-targeted FISH probes using Mathematical models