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General Analysis
→Complete Analysis for any probe-target pair including reaction ΔG
^{o}
's, ΔG
^{o}
overall, hybridization efficiency, and formamide dissociation profile.
ΔG
^{o}
_{overall}
Series
→ Calculate ΔG
^{o}
_{overall}
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
^{o}
_{1}
Series
→ Calculate ΔG
^{o}
_{1}
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
^{o}
_{2}
Series
→ Calculate ΔG
^{o}
_{2}
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
^{o}
_{3}
Series
→ Calculate ΔG
^{o}
_{3}
values for a series of probes of constant length targeting a given region on the organism of interest
Mismatch Analysis
→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
Formamide Curve Generator
→Derivation of formamide dissociation profiles for multiple probe/target pairs.
Competitor Analysis
→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.
Help
→ Guidelines for mathFISH
Links
→ Links to useful websites
Citations
→List of Citations
Contact Us
→ Give us your valuable feedback
mathFISH
:
A web tool for the computational evaluation of RNA-targeted FISH probes using
Mathematical
models