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General Analysis
→Complete Analysis for any probe-target pair including reaction ΔG
o
's, ΔG
o
overall, hybridization efficiency, and formamide dissociation profile.
ΔG
o
overall
Series
→ Calculate ΔG
o
overall
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
o
1
Series
→ Calculate ΔG
o
1
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
o
2
Series
→ Calculate ΔG
o
2
values for a series of probes of constant length targeting a given region on the organism of interest
ΔG
o
3
Series
→ Calculate ΔG
o
3
values for a series of probes of constant length targeting a given region on the organism of interest
Mismatch Analysis
→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
Formamide Curve Generator
→Derivation of formamide dissociation profiles for a given probe/target pair.
Competitor Analysis
→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.
Help
→ Guidelines for mathFISH
Links
→ Links to useful websites
Citations
→List of Citations
Contact Us
→ Give us your valuable feedback
CONTACT US
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E-mail address:
mathfish.cee.wisc.edu@gmail.com
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1415 Engineering Dr.
Civil and Environmental Engineering Department
UNIVERSITY OF WISCONSIN - MADISON
