• General Analysis→Complete Analysis for any probe-target pair including reaction ΔG^o's, ΔG^ooverall, hybridization efficiency, and formamide dissociation profile.
• ΔG^o_overall Series→ Calculate ΔG^o_overallvalues for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₁ Series→ Calculate ΔG^o₁values for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₂ Series→ Calculate ΔG^o₂values for a series of probes of constant length targeting a given region on the organism of interest
• ΔG^o₃ Series→ Calculate ΔG^o₃values for a series of probes of constant length targeting a given region on the organism of interest
• Mismatch Analysis→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
• Formamide Curve Generator→Derivation of formamide dissociation profiles for multiple probe/target pairs.
• Competitor Analysis→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.

• Help→ Guidelines for mathFISH
• Links→ Links to useful websites
• Citations→List of Citations
• Contact Us→ Give us your valuable feedback

ΔG^o_overall Series

  WARNING : This calculator takes worst case time of 2.5 minutes to come up with the results.

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Temperature:   ^oC          [ Na ⁺ ] :    M

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Enter the target frame :(?)Target frame is the fragment of the target where the oligonucleotide if the given length will be walked.

5' end:         3' end :       Probe Length :  

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Select your Target Molecule :  Small Subunit rRNA Large subunit rRNA Other

Select the Domain of life for the Target molecule :   Bacteria Archaea Eukarya

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Enter the sequence of the Target Organism :(?)Accepts plain text, GenBank and Fasta Format.