General Analysis→Complete Analysis for any probe-target pair including reaction ΔG^o's, ΔG^ooverall, hybridization efficiency, and formamide dissociation profile.
ΔG^o_overall Series→ Calculate ΔG^o_overallvalues for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₁ Series→ Calculate ΔG^o₁values for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₂ Series→ Calculate ΔG^o₂values for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₃ Series→ Calculate ΔG^o₃values for a series of probes of constant length targeting a given region on the organism of interest
Mismatch Analysis→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
Formamide Curve Generator→Derivation of formamide dissociation profiles for multiple probe/target pairs.
Competitor Analysis→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.

General Analysis

Temperature: ^oC [ Na ⁺]: M Probe Concentration : nM (?)Typical Range is 250nM to 1000nM.

Select Target Site Position Enter targetsite : 5' end : 3' end :
OR
Target Site Sequence Entry Target Site Sequence : 5' 3'
OR
Probe Sequence Entry Probe Sequence : 5' 3'

Select your Target Molecule :

Select the Domain of life for the Target molecule :

Enter the sequence of the Target Organism :(?) Accepts plain text, GenBank and Fasta Format. Gaps will be removed.